Cell Culture:Article Title: Salt and crystal form of modified andrographolide compound and preparation method thereof
Article Snippet: Materials: Cell line: THP-1 cell line THP-1 cell culture medium (RPMI 1640, Gibco #22400-089, 10% serum Gibco #10099-141) LPS, 1 mg/ml (Sigma #L5293) DPBS (Hyclone, #SH30028.01B) Human IL-6 CBA kit, BD #558276 CBA Human Soluble Protein Master Buffer Kit, BD #558265 Dexamethasone: J&K #308890 96-well cell plate, Corning # CO2 incubator, Thermo #371 Centrifuge, Eppendorf #5810R Vi-cell Cell Counter, Beckman Coulter FACSCalibur, BD #97500540 Experimental Procedures and Methods: a) Cell Plating 1) The medium was pre-heated in a 37° C. water bath; 2) The cell suspension in the culture flask was mixed well, transferred to a centrifuge tube, and centrifuged at 1200 rpm for 5 minutes at room temperature.
Article Title: Modified compound of andrographolide
Article Snippet: Experimental Materials: Cell line: THP-1 cell line THP-1 cell culture medium (RPMI 1640, Gibco #22400-089, 10% serum Gibco #10099-141) LPS, 1 mg/ml (Sigma # L5293) DPBS (Hyclone, # SH30028.01B) Human IL-6 CBA kit, BD #558276 CBA Human Soluble Protein Master Buffer Kit, BD #558265 Dexamethasone: J&K #308890 96-well Cell Plate, Corning # CO2 incubator, Thermo #371 Centrifuge, Eppendorf #5810R Vi-cell Cell Counter, Beckman Coulter FACSCalibur, BD #97500540 Experimental procedures and methods: a) Cell inoculation 1) The medium was preheated in a 37° C. water bath.
Crocin Bleaching Assay:Article Title: Salt and crystal form of modified andrographolide compound and preparation method thereof
Article Snippet: Materials: Cell line: THP-1 cell line THP-1 cell culture medium (RPMI 1640, Gibco #22400-089, 10% serum Gibco #10099-141) LPS, 1 mg/ml (Sigma #L5293) DPBS (Hyclone, #SH30028.01B) Human IL-6 CBA kit, BD #558276 CBA Human Soluble Protein Master Buffer Kit, BD #558265 Dexamethasone: J&K #308890 96-well cell plate, Corning # CO2 incubator, Thermo #371 Centrifuge, Eppendorf #5810R Vi-cell Cell Counter, Beckman Coulter FACSCalibur, BD #97500540 Experimental Procedures and Methods: a) Cell Plating 1) The medium was pre-heated in a 37° C. water bath; 2) The cell suspension in the culture flask was mixed well, transferred to a centrifuge tube, and centrifuged at 1200 rpm for 5 minutes at room temperature.
Article Title: Modified compound of andrographolide
Article Snippet: Experimental Materials: Cell line: THP-1 cell line THP-1 cell culture medium (RPMI 1640, Gibco #22400-089, 10% serum Gibco #10099-141) LPS, 1 mg/ml (Sigma # L5293) DPBS (Hyclone, # SH30028.01B) Human IL-6 CBA kit, BD #558276 CBA Human Soluble Protein Master Buffer Kit, BD #558265 Dexamethasone: J&K #308890 96-well Cell Plate, Corning # CO2 incubator, Thermo #371 Centrifuge, Eppendorf #5810R Vi-cell Cell Counter, Beckman Coulter FACSCalibur, BD #97500540 Experimental procedures and methods: a) Cell inoculation 1) The medium was preheated in a 37° C. water bath.
Suspension:Article Title: Salt and crystal form of modified andrographolide compound and preparation method thereof
Article Snippet: Materials: Cell line: THP-1 cell line THP-1 cell culture medium (RPMI 1640, Gibco #22400-089, 10% serum Gibco #10099-141) LPS, 1 mg/ml (Sigma #L5293) DPBS (Hyclone, #SH30028.01B) Human IL-6 CBA kit, BD #558276 CBA Human Soluble Protein Master Buffer Kit, BD #558265 Dexamethasone: J&K #308890 96-well cell plate, Corning # CO2 incubator, Thermo #371 Centrifuge, Eppendorf #5810R Vi-cell Cell Counter, Beckman Coulter FACSCalibur, BD #97500540 Experimental Procedures and Methods: a) Cell Plating 1) The medium was pre-heated in a 37° C. water bath; 2) The cell suspension in the culture flask was mixed well, transferred to a centrifuge tube, and centrifuged at 1200 rpm for 5 minutes at room temperature.
Article Title: Modified compound of andrographolide
Article Snippet: Experimental Materials: Cell line: THP-1 cell line THP-1 cell culture medium (RPMI 1640, Gibco #22400-089, 10% serum Gibco #10099-141) LPS, 1 mg/ml (Sigma # L5293) DPBS (Hyclone, # SH30028.01B) Human IL-6 CBA kit, BD #558276 CBA Human Soluble Protein Master Buffer Kit, BD #558265 Dexamethasone: J&K #308890 96-well Cell Plate, Corning # CO2 incubator, Thermo #371 Centrifuge, Eppendorf #5810R Vi-cell Cell Counter, Beckman Coulter FACSCalibur, BD #97500540 Experimental procedures and methods: a) Cell inoculation 1) The medium was preheated in a 37° C. water bath.
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